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微信小章| 产品名称： | SW10 |
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| 商品货号： | TS175692 |
| Organism： | Mus musculus, mouse |
| Cell Type： | neuronal Schwann cell; immortalized with SV40 large T antigen |
| Product Format： | frozen |
| Morphology： | neuronal |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Primary Schwann cells were infected with a retroviral vector carrying a temperature sensitive SV40 large T antigen. |
| Genes Expressed： | Myelin-associated glycoprotein (Mag); protein, negativexa0Ref Myelin basic protein (Mbp); protein, negativexa0Ref Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); protein, negativexa0Ref Peripheral myelin protein 22 (Pmp22); protein, negativexa0Ref S100 calcium-binding protein, beta (neural) (S100b); protein, positivexa0Ref |
| Comments： | The cells can be cultured at the permissive temperature of 33°C (proliferative) or the restrictive temperature of 37°C (nonproliferative).xa0Ref A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. Primary Schwann cells were infected with a retroviral vector carrying a temperature sensitive SV40 large T antigen.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 33°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Temperature Effects： | Restrictive temperature: 37°C yes
Permissive temperature: 33°C yes
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| Name of Depositor： | PI Patel |
| References： | Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843 |
Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843