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| 产品名称： | phi8-9 p8-9, phi 8-9 |
|---|---|
| 商品货号： | TS175760 |
| Designations： | phi8-9 p8-9, phi 8-9 |
| Species： | Homo sapiens, human |
| Applications： | Highly polymorphic locus with RFLPs observed with BclII, BglI, BglII, BstNI, MspI, PstI, PvuII, SacI and TaqI. Most seem to be site mutations. |
| Vector： | Construct size (kb): 46.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 18.0 Gene product: DNA Segment, single copy D16S20 Alleles: A1, B1, D1, B2, A2, D2 |
| Insert Size (kb)： | 18.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): 18.0 + arms. This probe has been assigned to a linkage group on 16q (Lathrop et al., HGM9). The order of marker loci is cen-HP-CTRB-D16S20-pLC9-(APRT,D16S7)-qter. Highly polymorphic locus with RFLPs observed with BclII, BglI, BglII, BstNI, MspI, PstI, PvuII, SacI and TaqI. Most seem to be site mutations. Sites A, B, and D show a heterozygosity of 86% (sample of 36 unrelated individuals). The whole phage works well as a probe after prehybridization with total human DNA to block annealing of repetitive sequences. The final stringency wash is 0.1X SSC/ 0.1% SDS at 65C. |
| References： | Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986 Maslen C, et al. An unusual clustering of restriction site polymorphisms at D16S20. Cytogenet. Cell Genet. 46: 656, 1987. Michael Litt, personal communication |