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| 产品名称： | AR.C |
|---|---|
| 商品货号： | TS175813 |
| Designations： | AR.C |
| GenBank Number： | M59856 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 0.0 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: aldehyde reductase 1 (low Km aldose reductase)(aldehyde reductase 1, low Km (aldose reductase)) ALDR1 |
| Insert Size (kb)： | 40.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--17.0, 7.4, 3.5, 3.2, 2.9; BglII--13.0, 7.8, 6.6 (doublet), 2.4, 2.1, 1.7, 1.55, 0.85, 0.7; HindIII--9.4, 6.6 (doublet), 5.6, 5.0, 4.0, 2.6, 2.05, 1.5, 0.9, 0.65; PvuII--11.0, 5.4, 4.2, 3.0, 2.3, 2.15, 2.05, 1.55, 1.1, 0.9, 0.7; SacI--10.0, 9.4, 5.8, 3.8, 3.1, 2.2, 1.8, 1.65, 1.45, 1.3, 1.0, 0.75; KpnI--20.0, 8.4, 7.8, 3.1. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Contains the complete gene which spans approximately 18 kb and includes 10 exons. GenBank M59856 records 5 flanking sequence, exon 1, and part of intron 1. GenBank M59783 begins about 2.3 kb downstream and includes the rest of the gene. |
| References： | Graham A, et al. Nucleotide sequence of c DNA for human aldose reductase. Nucleic Acids Res. 17: 8368, 1989. PubMed: 2510130 Graham A, et al. Structure of the human aldose reductase gene. J. Biol. Chem. 266: 6872-6877, 1991. PubMed: 1901857 Graham A, et al. The human aldose reductase gene maps to chromosome region 7q35. Hum. Genet. 86: 509-514, 1991. PubMed: 1901827 Alexander Graham, personal communication |