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| 产品名称： | pAS1-p53 |
|---|---|
| 商品货号： | TS175819 |
| Designations： | pAS1-p53 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein tumor protein p53 (Li-Fraumeni syndrome) tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pAS1 Intact vector size: 7.100 Type of vector: phagemid Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): promoter: ADC1 (ADH), -> other: GAL4 DNA binding domain, -> epitope tag: HA (hemagglutinin), -> restriction site: EcoRI, ->, 4315 MCS: NdeI...SalI, ->, 4395-4422 terminator: ADC1 replicon: pMB1 marker(s): ampR replicon: 2 micron marker(s): TRP1, -> replicon: f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: TP53 Genomic copy number: unique Gene name: tumor protein p53 (Li-Fraumeni syndrome) Contains complete coding sequence?: U Chromosome: 17; Localization: 17 p13.1 Type of DNA: cDNA Insert end: Insert end: Insert size (kb): Cross references: Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) TP53 Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2 Target Gene: tumor protein p53 (Li-Fraumeni syndrome) |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--6.0, 1.25, 0.95; BamHI/HindIII--5.0, 1.45, 0.95, 0.75; EcoRI-- 8.2; BamHI--8.2; PstI--7.2, 0.95. Used as a negative control in the 2-hybrid cloning system. |
| References： | Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 |