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| 产品名称： | Rhizobium radiobacter (Beijerinck and van Delden) Young et al. |
|---|---|
| 商品货号： | TS175856 |
| Deposited As： | Agrobacterium tumefaciens |
| Strain Designations： | NTL4(pZLR4) |
| Application： | Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals. |
| Isolation： | This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Type Strain： | no |
| Comments： | The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention.
Pzlr4 confers resistance to beta lactam antibiotics and gentamicin. The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity. |
| Medium： | ATCC® Medium 1885: LB medium (ATCC medium 1065) with 15 mcg/ml gentamycin |
| Growth Conditions： | Temperature: 28°C Atmosphere: Aerobic |
| Name of Depositor： | S Farrand |
| References： | Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399 |