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| 产品名称： | YIp367 |
|---|---|
| 商品货号： | TS175934 |
| Designations： | YIp367 |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: YIp367 (plasmid) Promoters: Promoter none Construction: YEp367 (ATCC 37735), YIp351 Marker(s):LEU2,ampR Construct size (kb): 7.0 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: none replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SacI-2; KpnI-2; SmaI-1; BamHI-1; XbaI-1; SalI-1; PstI-2; SphI-2; HindIII-1. The EcoRI, SacI, and KpnI sites are not unique. Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.9; SalI--7.0; PstI--7.0; HindIII--7.0. One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37752 - 37754) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5GAA TTC GAG CTC GGT ACC CGG GGA TCC TCT AGA GTC GAC CTG CAG GCA TGC AAG CTT GCT CCC 3, from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |