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| 产品名称： | pBP90C |
|---|---|
| 商品货号： | TS175995 |
| Designations： | pBP90C |
| Depositors： | RH Reeves |
| Biosafety Level： | 1 |
| Vector Information： | Vector: pBP90C (plasmid) Type: expression Marker(s):ampR,HIS3 |
| Applications： | YI-type (integrating) shuttle vector vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--9.3; HindIII--4.9, 2.55, 1.85; XbaI--uncut; NotI--9.3. YI-type shuttle integrative vector targeting human LINE sequences in YAC libraries derived from somatic cell hybrids. Constructed by replacing a 2.2 kb XbaI fragment of the LINE element in pL1.1a with a blunt-ended 1.2 kb fragment containing HIS3. The replaced element, nt 3392 to 5607 of the LINE consensus sequence, contained conserved sequences which might have permitted targeting to murine-derived YACs. Linearizing with NotI and ClaI removes plasmid sequences from the LINE ends for targeting integration. The NotI site is at the 5 end of the LINE sequence. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Pavan WJ, Reeves RH. Integrative selection of human chromosome-specific yeast artificial chromosomes. Proc. Natl. Acad. Sci. USA 88: 7788-7791, 1991. PubMed: 1881915 Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105 |
| Shipped： | freeze-dried |