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微信小章| 产品名称： | Scheffersomyces shehatae (Buckley et van Uden) Urbina et Blackwell |
|---|---|
| 商品货号： | TS176094 |
| Deposited As： | Candida shehatae Buckley et van Uden, anamorph |
| Strain Designations： | M 175 NRC 2883 |
| Application： | Bioethanol production |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Comments： | Ferments xylose Increase in ploidy during stress
Genome sequencing strain (Nanjing Forestry University, China) |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCGGCGAAAAAACCTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTTAGAAATAAGTTGGGCCAGAGGTTAACCAAACTTCAATTTTATTATTGAATTGTTATTTTATTTAATTTGTCAATTTGTTGATTAAATTCAAAAAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAGCCCTCGGGTTTGGTATTGAGTGATACTCTTAGTCAGACTAGGCGTTTGCTTGAAAAGTATCGGCATGAGTAGTACTAGATAGTGCTTTCAGGATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGAATTCTTGGTAGTGAATTTTTAGTATCATGGCTCTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGTCTTAGTAACGGCGAGTGAAGCGACAAAAGCTCAAATTTGAAATCTGGCACCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTTGGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCAACTCTATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGGGCCTCTGCAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTGGCACTACTTCGGTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCCTAAACCGC |
| Name of Depositor： | SA Meyer |
| Cross References： | Nucleotide (GenBank) : JQ023135 RPB 1 gene Nucleotide (GenBank) : HQ652024 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : HQ652065 ITS including 5.8S rRNA gene Nucleotide (GenBank) : JQ023147 RPB 2 gene Nucleotide (GenBank) : KC479677 18S rRNA gene Nucleotide (GenBank) : KC507458 EF-1 alpha gene Nucleotide (GenBank) : KC479711 XYL1 gene |
| References： | Alexander MA, et al. Levels of pentose phosphate pathway enzymes from Candida shehatae grown in continuous culture. Appl. Microbiol. Biotechnol. 29: 282-288, 1988. Hinfray CT, et al. Influence of the oxygenation level on D-xylose fermentation by free and agar-entrapped cultures of C. shehatae. Appl Microbiol Biotechnol 42: 682-687, 1995 Jeffries TW. Effects of culture conditions on the fermentation of xylose to ethanol by Candida shehatae. Biotechnol. Bioeng. Symp. 15: 149-166, 1985. Lee H, Fisher BG. Unusual fructose utilization by Pichia stipitis and its potential application. J. Ferment. Bioeng. 69: 79-82, 1990. Palnitkar S, Lachke A. Effect of nitrogen sources on oxidoreductive enzymes and ethanol production during D-xylose fermentation by Candida shehatae. Can. J. Microbiol. 38: 258-260, 1992. PubMed: 1393828 Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624 Talbot NJ, Wayman M. Increase in ploidy in yeasts as a response to stressing media. Appl Microbiol Biotechnol 32: 167-169, 1989 Yang VW, Jeffries TW. Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae. Appl Biochem Biotechnol 26: 197-206, 1990 |