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微信小章| 产品名称： | Trichoderma afroharzianum Chaverri et al. |
|---|---|
| 商品货号： | TS176197 |
| Deposited As： | Trichoderma harzianum Rifai |
| Strain Designations： | 1295-22 T-22 |
| Application： | Biological control of Fusarium graminearum
Biological control of Rhizoctonia solani
Biological control of Sclerotium rolfsii
Biological control of Pythium ultimum
Promotes plant growth and vigor and improves the efficiency of root systems |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Preceptrol®： | no |
| Comments： | The strain was recently re-identified as Trichoderma afroharzianum, a close sister of Trichoderma harzianum, based on the multigene sequence analysis. |
| Morphology： | On PDA medium at 25°C after 8 days, mycelium white becoming green to dark green, tomentose. Reverse reddish brown. Hyphae hyaline, guttulate. Conidiophores with phialides in whorls of 3-4. Phialides 9-10.5 X 2.5-3 µm. Conidia green, globosa to subglobose, smooth, 3-6 X 2.5-5.5 µm. Chlamydospores terminal and intercalary. |
| Medium： | ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 25°C Atmosphre: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTATTGTATACCCCCTCGCGGGTTTTTTTATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTGCCTTGGCGGTGGCCGTCTCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCTAGGGCCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCCGCCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACCTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGGGCGCGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTCGCCGCGTCTAGGCCAGCATCAGTTCGTCGCGGGGGAAAAAGGCTTCGGGAACGTGGCTCCTCCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGGTGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCACCAGCGAC elongation factor (EF) 1-alpha gene CACTGGTACTTCCCAGGCCGATTGCGCTATCCTCATCATTGCCGCCGGTACTGGTGAGTTCGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCACGCTCTGCTCGCCTACACCCTGGGTGTTAAGCAGCTCATCGTTGCCATCAACAAGATGGACACTGCCAACTGGGCCGAGGCTCGTTACCAGGAAATCATCAAGGAGACTTCCAACTTCATCAAGAAGGTCGGCTTCAACCCCAAGGCTGTTGCTTTCGTCCCCATCTCCGGTTTCAACGGTGACAACATGCTCCAGCCCTCCACCAACTGCCCCTGGTACAAGGGCTGGGAGAAGGAGACCAAGGCTGGCAAGTTCACCGGCAAGACCCTCCTTGAGGCCATCGACTCCATCGAGCCCCCCAAGCGTCCCACGGACAAGCCCCTCCGTCTTCCCCTCCAGGATGTCTACAAGATCGGTGGTATTGGAACAGTTCCCGTCGGCCGTATCGAGACTGGTGTCCTCAAGCCCGGTATGGTCGTCACTTTCGCTCCCTCCAACGTCACCACTGAAGTCAAGTCCGTCGAGATGCACCACGAGCAGCTCGTCGAGGGTGTTCCCGGTGACAACGTTGGTTTCAACGTCAAGAACGTTTCCGTTAAGGAAATTCGCCGTGGTAACGTTGCCGGTGACTCCAAGAACGACCCCCCCATGGGTGCCGCTTCTTTCACCGCTCAGGTCATCGTCATGAACCACCCTGGCCAGGTCGGTGCCGGCTACGCCCCCGTTCTTGACTGCCACACTGCCCACATTGCCTGCAAGTTCGCCGAGCTCCAGGAGAAGATCGACCGCCGTACCGGTAAGGCTACCGAGACTGCCCCCAAGTTCATCAAGTCCGGTGACTCTGCCATCGTCAAGATGATTCCCTCCAAGCCCATGTGCGTTGAGGCTTTCACCGACTACCCTCCCCTGGGTCGTTTCGCCGTCCGTG calmodulin gene TCTTTGTGAGTCTTTAACCTCGTTTCTGGTGGCAATTCGGACGCTTTGAGTGCTAACCCAGCTGTTACAGGACAAGGACGGTGATGGTACGTAGCAGCGAGTGACGCGACACCACCCCTCTCCCCCCTTTGCGAACAGGCACCGGAGCACCGAATGACTTGCTTCCGACCCATATCTTCCTTGACAACCCCTTTTCATGATATCTGAGCAAAGAAACGAACTGACAGGCTTGACCCCGCAGGCCAGATCACCACCAAGGAGCTGGGCACCGTGATGCGCTCCCTCGGCCAGAACCCCTCCGAGTCAGAGCTGCAGGACATGATTAACGAGGTCGATGCCGACAACAACGGATCTATCGATTTCCCCGGTATGTCAAAAGTCAAAGTGTTGGGTCGTTCCCATATTCGAGCTAATACGGAGCGGTGTAGAATTCCTGA |
| Name of Depositor： | Cornell Res. Fndn., Inc. |
| U.S. Patent Number： | |
| Isolation： | Protoplast fusion of ATCC® 20737™ and ATCC® 60850™ |
| Cross References： | Nucleotide (GenBank) : FJ545255 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU933424 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : KU933430 elongation factor (EF) 1-alpha gene Nucleotide (GenBank) : KU933406 calmodulin gene |
| References： | Altomare C, et al. Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Appl. Environ. Microbiol. 65: 2926-2933, 1999. PubMed: 10388685 Lo CT, et al. Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology 88: 129-136, 1998. Stasz TE, et al. Fused biocontrol agents. US Patent 5,260,213 dated Nov 9 1993 |