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| 产品名称： | CHON-002 |
|---|---|
| 商品货号： | TS176215 |
| Organism： | Homo sapiens, human |
| Tissue： | Long bone; cartilage |
| Cell Type： | Fibroblast immortalized with hTERT |
| Product Format： | frozen |
| Morphology： | Fibroblast-like |
| Culture Properties： | Adherent |
| Biosafety Level： | 2 Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | Fetal, 18 weeks |
| Gender： | Female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a diploid cell line of female origin. Overall, the karyology is stable with a modal chromosome number of 46 in 87% of the examined cells and a low rate of polypoidy. No consistent structural chromosomal aberrations were found in any of the cells examined. |
| Images： |  |
| Derivation： | The chondrocyte cell line, CHON-002, was derived from the long bones of an 18-week female fetus. The primary cells were infected by the defective retrovirus containing hTERT gene under G418 selection. The defective retrovirus was collected from the supernatant of the packaging cell line, PT67 transfected with the pLXSN vector containing hTERT gene.
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| Antigen Expression： | This cell line expresses the following (Homo sapiens) proteins: HLA-B81,xa0HLA-B58,xa0HLA-Cw6,xa0HLA-Cw8,xa0HLA-DR7,xa0HLA-DR12,xa0HLA-DR52,xa0HLA-DQ2,xa0HLA-DQ5. |
| Genes Expressed： | Positive for Collagen type I and aggrecan (RT-PCR). Negative for Collagen type II (RT-PCR). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes are given for T-75 flasks; proportionally reduce or increase the amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Aminal Cells: A Manual of Basic Techniques by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Freeze medium: 90% heat-inactivated fetal bovine serum; 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 12,14 D16S539: 11,13 D5S818: 11,12 D7S820: 12 THO1: 6,7 TPOX: 8,11 vWA: 16,17 |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor： | ATCC |
| Year of Origin： | December 20, 2001 |
| References： | Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13. |