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| 产品名称： | c8179 |
|---|---|
| 商品货号： | TS176317 |
| Designations： | c8179 |
| GenBank Number： | U00062 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 47.45000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 41.01900100708008 Gene product: pyrroline-5-carboxylate dehydrogenase AAP1 |
| Insert Size (kb)： | 41.019 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 8.595, 0.427 (vector); 5.15, 4.26, 3.364, 2.932, 2.521, 1.903, 1.786, 1.687, 1.62, 1.281, 1.225, 1.011, 0.965, 0.936, 0.933, 0.904, 0.831, 0.780, 0.763, 0.700, 0.649. 0.471. 0.427. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 168828-209847 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |