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| 产品名称： | pHC79 |
|---|---|
| 商品货号： | TS176318 |
| Designations： | pHC79 |
| Depositors： | J Collins |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.43 Vector: pHC79 (cosmid) Construction: pBR322, Charon 4A Marker(s):ampR,tetR Construct size (kb): 6.43 Features: marker(s): ampR, tetR replicon: pMB1 terminator: none |
| Applications： | vector for constructing cDNA libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. General purpose cosmid vector derived from pBR322. pBR322 was partially digested with Sau3A and ligated with an 0.65 BglII lambda fragment, generating a BglII site (pBR322 positions 1459-1666 deleted). A 1.65 kb BglII fragment from Charon 4A was ligated into the BglII site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Hohn B, Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene 11: 291-298, 1980. PubMed: 6260575 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried stock of Escherichia coli containing the plasmid. |