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| 产品名称： | Choanoeca perplexa Ellis |
|---|---|
| 商品货号： | TS176329 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Atlantic Ocean off Plymouth, England, 1977 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 |
| Medium： | ATCC® Medium 1361: Marine flagellate medium |
| Growth Conditions： | Temperature: 15.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCC medium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCC medium 1361 in a T-25 flask. |
| Subcultivation： | Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCC medium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCC medium 1361 in a T-25 flask. |
| Name of Depositor： | BS Leadbeater |
| Year of Origin： | 1977 |
| References： | Leadbeater BS. . J. Mar. Biol. Assoc. U.K. 57: 285-301, 1977. Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774 |