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| 产品名称： | pAngC |
|---|---|
| 商品货号： | TS176411 |
| Designations： | pAngC |
| Species： | Homo sapiens, human |
| Depositors： | BL Vallee |
| Applications： | The ApaI fragment extending from nt 58 in the pIBI25 polylinker to nt 569 in the 3 noncoding region has been used as a probe. |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 0.7260000109672546 Gene product: angiogenin ANG Alleles: A1, A2 |
| Insert Size (kb)： | 0.726 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.7; EcoRI--3.4; HindIII--3.4; PstI--3.4; BamHI--3.4. The ApaI fragment extending from nt 58 in the pIBI25 polylinker to nt 569 in the 3 noncoding region has been used as a probe. This contains the complete 441 bp coding sequence as well as portions of the 5 (44 bp) and 3 (15 bp) noncoding regions. The coding sequence extends from nt 113 to nt 554 of the insert. Nucleotides 1-69 are derived from the pIBI25 polylinker. The AvaII polymorphism results from a silent transversion at the third position of codon +86. Derived from a plasmid constructed by cloning a genomic AccI fragment into the HincII site of pIBI25. The EcoRV insert thus contains some sequences at the 5 end derived from the pIBI25 polylinker. |
| References： | Weremowicz S, et al. Localization of the human angiogenin gene to chromosome band 14q11, proximal to the T cell receptor alpha/delta locus. Am. J. Hum. Genet. 47: 973-981, 1990. PubMed: 1978563 Bert L Vallee, personal communication |