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| 产品名称： | pBLV666 |
|---|---|
| 商品货号： | TS176425 |
| Designations： | pBLV666 |
| GenBank Number： | K02120 |
| Species： | Bovine leukemia virus |
| Depositors： | MB Johnson, JP Mone |
| Vector： | Construct size (kb): 3.624000072479248 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6660000085830688 Gene product: bovine leukemia virus capsid protein gag-p24* |
| Insert Size (kb)： | 0.666 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--3.1, 0.5; HindIII--3.6; BamHI/PvuII--2.5, 0.8, 0.25. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.15, 0.61; SacII--0.59; SalI--0.21. The insert gene was cloned by PCR amplification of a provirus isolated from a naturally infected cow. The insert contains artificially introduced start and stop codons flanking the coding region. The insert also contains a BamHI site, immediately upstream from the start codon and a PvuII site, immediately downstream from the ochre stop codon introduced by PCR. The Pfu polymerase-generated blunt end PCR product was cloned into the EcoRV site of pBluescript SK+ and the EcoRV site was destroyed after cloning. |
| Classification： | Retrovirus, HTLV-BLV |