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| 产品名称： | hPAR2 cDNA |
|---|---|
| 商品货号： | TS176430 |
| Designations： | hPAR2 cDNA |
| GenBank Number： | Z49993 Z49994 |
| Species： | Homo sapiens, human |
| Depositors： | JB Sundelin |
| Applications： | in another host, produces protein G-protein coupled receptor 11 proteinase-activated receptor 2 |
| Vector： | Construct size (kb): 5.360000133514404 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSG5 Intact vector size: 4.076 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI BamHI Polylinker sites: EcoRI BamHI BglII Construction: pKCR2, Bluescribe M13+ Host range: eukaryotic cells; Escherichia coli Features (with orientation and position when available): restriction site: SalI promoter for expression: SV40 early, -> other: rabbit beta-globin intron II, -> promoter for in vitro transcription: T7, -> MCS: EcoRI...BglII, -> terminator: SV40 polyadenylation, -> replicon: pMB1, marker(s): ampR, replicon: M13, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GPR11 Other gene symbols: PAR2* Gene name: G-protein coupled receptor 11 Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q13 Tissue: stomach Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 1.283 Cross references: DNA Seq. Acc.: Z49993 Nucleotides 1-91 of the insert correspond to nucleotides 195-285 of Z49993. DNA Seq. Acc.: Z49994 Nucleotides 92-1283 of the insert correspond to nucleotides 97-1288 of Z49994. Insert lengths(kb): 1.282999992370606 Tissue: stomach Gene product: G-protein coupled receptor 11(proteinase-activated receptor 2) GPR11 Target Gene: G-protein coupled receptor 11 |
| Insert Size (kb)： | 1.283 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 1.3; EcoRI--5.4; BglI--2.1, 1.6, 1.1, 0.45. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.35; NcoI--0.55, 0.83; PstI--0.07; PvuII--1.02. Insert was amplified by PCR of total RNA from human stomach, followed by restriction digestion with BamHI. |
| References： | Nystedt S, et al. Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor2. Eur. J. Biochem. 232: 84-89, 1995. PubMed: 7556175 |