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| 产品名称： | pHR81 |
|---|---|
| 商品货号： | TS176444 |
| Designations： | pHR81 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Vector: pHR81 (phagemid) Construction: pUC119 Marker(s):ampR Features: marker(s): URA3 marker(s): ampR other: leu2-d replicon: pMB1, M13, 2 micron ori |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--7.0, 1.2; XbaI--8.2; PstI--8.2. The LEU2-d and 2 micron sequences are between the unique cloning sites, and thus the adjacent SacI, BamHI, and XbaI sites can be used for directional cloning, and similarly the SphI and SalI sites can be used. Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. One of 9 YE-type shuttle vectors (TS176444, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine. Vector was constructed by inserting 1) URA3 HindIII fragment into HindIII site; 2) a SpeI fragment containing 2 micron ori and 3) the leu2-d gene into the XbaI site of pUC119. Both inserted yeast genes have the opposite orientation of the ampR gene. |
| References： | Nehlin JO, et al. Yeast galactose permease is related to yeast and mammalian glucose transporters. Gene 85: 313-319, 1989. PubMed: 2697639 Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397 |
| Shipped： | freeze-dried |