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| 产品名称： | pJC20 plasmid in E. coli |
|---|---|
| 商品货号： | TS176507 |
| Designations： | pJC20 plasmid in E. coli |
| Depositors： | J Clos |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.3399999141693120 Vector: pJC20 plasmid in E. coli (plasmid) Promoters: Promoter for expression T7 (phi10) Construction: pJC10 Marker(s):ampR Construct size (kb): 2.339999914169312 Features: marker(s): ampR promoter for expression: T7 (phi10) replicon: pMB1 MCS: NdeI...ApaI ribosome-binding site: Shine-Dalgarno terminator: T phi |
| Applications： | expression vector vector for generating nested deletions of cloned inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.3; HindIII--2.3; XbaI--2.3. High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Clos J, Brandau S. pJC20 and pJC40--two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli. Protein Expr. Purif. 5: 133-137, 1994. PubMed: 8054844 Clos J, et al. Molecular cloning and expression of hexameric Drosophila heat shock factor subject to negative regulation. Cell 63: 1085-1097, 1990. PubMed: 2257625 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried plasmid in E. coli |