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| 产品名称： | pHuPP4.6 |
|---|---|
| 商品货号： | TS176532 |
| Designations： | pHuPP4.6 |
| GenBank Number： | X13993 |
| Species： | Homo sapiens, human |
| Depositors： | G Safrany |
| Vector： | Construct size (kb): 7.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.599999904632568 Tissue: HeLa cell line Gene product: RNA, ribosomal, non-transcribed spacer RNR@* |
| Insert Size (kb)： | 4.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; BamHI--7.3; HindIII--7.3; PstI--4.4, 2.7; EcoRI/HindIII--4.4, 2.7. This clone is difficult to maintain, with deletions and/or viability loss with prolonged storage on LB agar. The depositor recommends storing as DNA. The fragment begins at nt 1285 of the HXP sequence (EMBL X13993). Downstream from the HXP sequence there is about a 3.0 kb long unsequenced gap with no known restriction sites, then the fragment continues and ends with the XXP sequence (EMBL X13991). This PstI fragment includes a 3.1 kb PstI/XbaI fragment with no known restriction sites. The 5 portion of the 3.1 sequence is A-rich; followed by a GC-rich region, and a 3 pyrimidine-rich sequence composed mainly of CT dinucleotides. The 4.3 kb XbaI repeat occurs in 2 copies in a 10.3 kb HindIII fragment (HeLa cells) of the non-transcribed spacer of ribosomal RNA. The insert contains Alu type repeats. |
| References： | Safrany G, Hidvegi EJ. New tandem repeat region in the non-transcribed spacer of human ribosomal RNA gene. Nucleic Acids Res. 17: 3013-3022, 1989. PubMed: 2726449 Geza Safrany, personal communication |