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微信小章| 产品名称： | 2B8 |
|---|---|
| 商品货号： | TS176538 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphocyte; transfected with plasmid pSV2ne |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain EBV particles
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 7 years |
| Ethnicity： | Black, African |
| Applications： | The 2B8 cell line (TS176538) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitts lymphoma cell line (ATCC HTB-62). |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | The 2B8 cell line (TS176538) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitts lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line. |
| Clinical Data： | Black, African 7 years |
| Comments： | The 2B8 cell line (TS176538) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitts lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Bayer Corporation |
| U.S. Patent Number： | |
| References： | Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |