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微信小章| 产品名称： | Trichoderma atroviride Karsten sensu Bissett |
|---|---|
| 商品货号： | TS176609 |
| Deposited As： | Trichoderma harzianum Rifai |
| Strain Designations： | 107 P1 P1 |
| Alternate State： | Hypocrea atroviridis Dodd et al. |
| Application： | Produces chitin 1,4-beta-chitobiosidase chitobiosidase
Produces endo-1,3(4)-beta-glucanase endo-1,3-beta-glucanase, laminarinase
Produces endochitinase
Produces glucan 1,3-beta-glucosidase beta-D-1,3-glucanase, exo-1,3-beta-glucanase, exo-1,3-beta-glucosidase
Produces hexosaminidase A (alpha polypeptide) Hexose aminidase A (alpha polypeptide), beta-N-acetylhexosaminidase, N-acetyl-beta-glucosaminidase
Biological control of Pythium spp.
Biological control of Botrytis cinerea
Produces a hydrophin-like protein in aerial spores |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Preceptrol®： | no |
| Comments： | Conidia that have already germinated at the time of application are better able to inhibit Botrytis cinerea than those that have not germinated, especially at low temperatures.
chitinase gene expression
ech42 gene expression before mycoparasitic contact |
| Medium： | ATCC® Medium 323: Malt agar medium ATCC® Medium 325: Malt extract agar (Blakeslees formula) ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACCATACCAAACTGTTGCCTCGGCGGGGTCACGCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGGACCAACCAAACTCTTTTCTGTAGTCCCCTCGCGGACGTTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGACCTCGGGAGCCCCTAAGACGGGATCCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTAGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGACGCCGCAGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACCTTGAAAAGAGGGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGGGCGCGGCGGATCATCCGGGGTTCTCCCCGGTGCACTTCGCCGTGTTCAGGCCAGCATCAGTTCGGCGCGGGGGAAAAAGGCTTCGGGAACGTGGCTCCTCCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGCTGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCACCAGCGA |
| Name of Depositor： | Cornell Res. Fndn., Inc. |
| U.S. Patent Number： | |
| Special Collection： | NCRR Contract |
| Isolation： | Wood chips |
| Cross References： | Nucleotide (GenBank) : U24158 cDNA clone Th1433 Nucleotide (GenBank) : L14614 Trichoderma harzianum endochitinase mRNA, complete cds. Nucleotide (GenBank) : S83231 nag1=N-acetyl-beta-D-glucosaminidase, genomic DNA, 3060 nt Nucleotide (GenBank) : AF049074 Trichoderma harzianum internal transcribed spacer 1, partial sequence |
| References： | Lorito M, et al. Synergistic interaction between fungal cell wall degrading enzymes and different antifungal compounds enhances inhibition of spore germination. Microbiology 140: 623-629, 1994. PubMed: 8012584 Harman GE, et al. Antifungal synergistic combination of enzyme fungicide and non-enzymatic fungicide and use thereof. US Patent 5,433,947 dated Jul 18 1995 Muñoz GA, et al. Comparison of aerial and submerged spore properties for Trichoderma harzianum. FEMS Microbiol Lett 125: 63-70, 1995 Lorito M, et al. Chitinolytic enzymes produed by Trichoderma harzianum: antifungal activity of purified endochitinase and chitobiosidase. Phytopathology 83: 302-307, 1993. Lorito M, et al. Purification, characterization, and synergistic activity of a glucan 1,3-beta-glucosidase and an N-acetyl-beta-glucosaminidase from Trichoderma harzianum. Phytopathology 84: 398-405, 1994. Klemsdal SS, et al. Isolation and characterization of a cDNA from Trichoderma harzianum P1 encoding a 14-3-3 protein homolog. Gene 171: 123-127, 1996. PubMed: 8675020 Zeilinger S, et al. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet. Biol. 26: 131-140, 1999. PubMed: 10328983 Mach RL, et al. Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. Appl. Environ. Microbiol. 65: 1858-1863, 1999. PubMed: 10223970 Kuling C, et al. Enzyme diffusion from Trichoderma atroviride (= T. harzianum P1) to Rhizoctonia solani is a prerequisite for triggering of Trichoderma ech42 gene expression before mycoparasitic contact. Appl. Environ. Microbiol. 66: 2232-2234, 2000. PubMed: 10788407 Thrane C, et al. Substrate colonization, strain competition, enzyme production in vitro, and biocontrol of Pythium ultimum by Trichoderma spp. isolates P1 and T3. Eur. J. Plant Pathol. 106: 215-225, 2000. Hjeljord LG, et al. Antagonism of nutrient-activated conidia of Trichoderma harzianum (atroviride) P1 against Botrytis cinerea. Phytopathology 91: 1172-1180, 2001. |