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微信小章| 产品名称： | UACC-2087 |
|---|---|
| 商品货号： | TS176610 |
| Organism： | Homo sapiens, human |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 51 years old |
| Gender： | female |
| Applications： | The cells have been used in studies of p53 mutation, as well as the relegation of p53 on DSC3 and MAPSIN.
Ref   Watts GS, et al. The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells. Neoplasia 6(3): 187-194, 2004. PubMed: 15153330 xa0RefOshiro MM, et al. Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 22(23): 3624-3634, 2003. PubMed: 12789271 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | range=48-67; modal number=60-64 |
| Receptor Expression： | epidermal growth gactor receptor (EGFR), expressed neu (ErbB2; HER2), not expressed estrogen receptor (ER), not expressed progesterone receptor (PR), not expressed |
| Comments： | In addition, p53 V216M mutation was found in this cell line. The cells have been used in studies of p53 mutation, as well as the relegation of p53 on DSC3 and MAPSIN
Ref
Watts GS, et al. The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells. Neoplasia 6(3): 187-194, 2004. PubMed: 15153330 xa0RefOshiro MM, et al. Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 22(23): 3624-3634, 2003. PubMed: 12789271UACC-2087 was derived from a 51 year-old female with breast cancer. The patient underwent chemotherapy. This cell line is a triple negative breast cancer cell line. Estrogen receptors, progesterone receptors and HER-2/neu are negative in the cells. The expression of tumor suppressor MAPSIN and cell adhesion molecules DSC3 is also negative in this cell line.
Ref Domann FE, et al. Epigenetic silencing of maspin gene expression in human breast cancers. Int. J. Cancer. 85(6): 805-810, 2000 PubMed: 10709100xa0RefOshiro MM, et al. Epigenetic silencing of DSC3 is a common event in human breast cancer. Breast Cancer Res. 7(5): R669-R680, 2005. PubMed: 16168112 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Note: This cell line grows slowly. Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. Medium renewal: every 3 to 4 days |
| Cryopreservation： | Freeze medium: complete growth medium, 80%; FBS, 10%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 100% |
| Name of Depositor： | K Brown |
| References： | Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046 Watts GS, et al. The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells. Neoplasia 6(3): 187-194, 2004. PubMed: 15153330 Domann FE, et al. Epigenetic silencing of maspin gene expression in human breast cancers. Int. J. Cancer. 85(6): 805-810, 2000 PubMed: 10709100 Oshiro MM, et al. Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 22(23): 3624-3634, 2003. PubMed: 12789271 Futscher BW, et al. Chapter 6: Epigenetic Dysregulation of Maspin (SerpinB5) in Cancer Invasion and Metastasis. DNA Methylation, Epigenetics and Metastasis. Cancer Metastasis - Biology and Treatment, 7: 133-155, 2005. Oshiro MM, et al. Epigenetic silencing of DSC3 is a common event in human breast cancer. Breast Cancer Res. 7(5): R669-R680, 2005. PubMed: 16168112 Futscher BW, et al. Chapter 7: Epigenetic Regulation of Genes That Affect Tumor Cell Adhesion. Cell Adhesion and Cytoskeletal Molecules in Metastasis. Cancer Metastasis - Biology and Treatment, 9: 123-140, 2006. Robey IF, et al. Regulation of the warburg effect in early-passage breast cancer cells. Neoplasia 10(8): 745-756, 2008. PubMed: 18670636 |