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| 产品名称： | lambdaZd35 |
|---|---|
| 商品货号： | TS176676 |
| Designations： | lambdaZd35 |
| Depositors： | AJ Murphy |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 43.7000007629394500 Vector: lambdaZd35 (phage, lambda - replacement) Promoters: Promoter Mo-MLV Construction: Charon 30; pZIP-neoSV(x)1 Marker(s):G418R,ampR,kanR,neoR Construct size (kb): 43.70000076293945 Features: marker(s): ampR, kanR, neoR, G418R promoter: Mo-MLV, polyoma replicon: lambda terminator: 3 LTR |
| Applications： | expression vector lambda vector allowing conversion to plasmid shuttle vector vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25. A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817. The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in Escherichia coli RRI(pRK248), ATCC 47036. Allows expression of cloned cDNA in mammalian cells; can be used for transduction. Allows plasmid replication in mouse (WOP) and monkey (COS) cells. Cloned cDNA expressed from LTR promoter; neo expressed from polyoma promoter; no splicing site; ori of SV40 and pBR322. |
| References： | Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791 |
| Shipped： | freeze-dried |