宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pH52 [PH52, ph52]

货号 TS176710
中文名称 null
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产品名称: pH52 PH52, ph52
商品货号: TS176710
Designations: pH52 PH52, ph52
GenBank Number:

X15187

Species: Homo sapiens, human
Depositors: PK Srivastava
Vector:
Construct size (kb): 4.28000020980835
Insert:
DNA: cDNA
Insert lengths(kb): 1.080999970436096
Tissue: teratocarcinoma
Gene product: tumor rejection antigen (gp96) 1(tumor rejection antigen gp96) TRA1
Insert Size (kb): 1.081
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6, 1.0; HindIII--6.6; EcoRI/HindIII--3.0, 2.6, 1.0; BglI--2.4, 1.8, 1.2, 0.6 + smaller; PstI--3.0, 1.9, 1.5.
The following enzymes do not cut within the insert: AatI, AatII, AvaI, BglII, EcoRI, EcoRV, HaeII, HindIII, PvuII, SalI, SstI, XbaI, XhoI.
The insert contains the following restriction sites (nucleotide position according to the published sequence): BamHI--30; BglI--109; KpnI--599, PstI--1038.
This clone has a 2.6 kb EcoRI/HindIII fragment of unknown origin. Our EcoRI digest is consistent with deposited DNA.
The insert extends from nucleotide 13 to 1094 of the published sequence. The coding sequence extends from 106 to 2517, with the signal peptide encoded from 106 to 168.
References:

Maki RG, et al. Human homologue of murine tumor rejection antigen gp96: 5-regulatory and coding regions and relationship to stress-induced proteins. Proc. Natl. Acad. Sci. USA 87: 5658-5662, 1990. PubMed: 2377606

Pramod K Srivastava, personal communication