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| 产品名称： | pSV2-cat |
|---|---|
| 商品货号： | TS176748 |
| Designations： | pSV2-cat |
| Depositors： | BH Howard |
| Other IDs： | Nucleotide (GenBank) : VB0065 pSV2-Cat cloning vector. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1 Vector: pSV2-cat (plasmid) Promoters: Promoter SV40 Construction: pSV2-betaG, Tn9 Marker(s):ampR,cmlS Construct size (kb): 5.1 Features: marker(s): ampR, cmlS promoter: SV40 replicon: pMB1 |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the vector give the following sizes (kb): HindIII--5.0; BamHI--5.0; BamHI/HindIII--3.5, 1.6; AccI--5.0. Expresses chloramphenicol acetyltransferase in eukaryotic cell lines under the control of the simian virus 40 early promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240 Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651 Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985 |
| Shipped： | freeze-dried |