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| 产品名称： | pWR3.26 molecular clone of rhinovirus 14 purified plasmid DNA |
|---|---|
| 商品货号： | TS176825 |
| Designations： | pWR3.26 molecular clone of rhinovirus 14 purified plasmid DNA |
| GenBank Definition： | Human rhinovirus type 14 polyprotein gene. |
| GenBank Number： | L05355 |
| Species： | Human rhinovirus 14, Human rhinovirus 14 |
| Depositors： | W Lee |
| Applications： | contains sequence |
| Vector： | Type of vector:xa0 E. coli Name of vector:xa0 pUC-19 Intact vector size (kb):xa0 unknown at this time Markers (ie. AMPR):xa0 AMPR Cloning site:xa0 The HRV14 cDNA was transplanted into the vector at the StuI site, then additional modifications were made to the viral sequence by site-directed mutagenesis. (See reference article) |
| Insert： | Insert description (full-length, etc.): full-length HRV 14cDNA and T7 promoter
Insert size (kb):xa0 7.212 kb
Full/partial sequence available (ie. GenBank #): L05355 (viral sequence)
Markers (genes and/or restriction sites):
HindIII - 3 sites; Fragment lengths: 5338, 2362, 2147
MluI- 1 site; Fragment length: 9847 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The construct contains a full-length Human Rhinovirus type 14 (HRV14) genome cloned into the StuI site of the pMJ3 vector. HRV14 cDNA was transplanted into the vector at the StuI site. The 5 end of the cDNA is two bases (GG) from the transcription initiation site of the T7 promoter. A unique MluI site (ACGCGT) was added after the polyA tail. Full length transcripts can be produced using T7 RNA polymerase. T7 transcripts are half as infective as genomic RNA from virons. |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus B |
| References： | Lee WM, et al. Role of maturation cleavage in infectivity of picornaviruses: activation of an infectosome. J. Virol. 67: 2110-2122, 1993. PubMed: 8383233 |