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| 产品名称： | pHT10 |
|---|---|
| 商品货号： | TS176848 |
| Designations： | pHT10 |
| Species： | Moloney murine sarcoma virus, Moloney murine sarcoma virus |
| Depositors： | GF Van de Woude |
| Vector： | Construct size (kb): 6.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 2.099999904632568 Gene product: Moloney murine sarcoma viral (v-mos) oncogene homolog MOS Alleles: B2, B1 |
| Insert Size (kb)： | 2.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.0; EcoRI--6.4; PvuII--3.8, 2.6; BamHI--6.4. Very low transformation efficiency because lacks LTR. |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| References： | Blair DG, et al. Biological activity of cloned Moloney sarcoma virus DNA: Terminally redundant sequences may enhance transformation efficiency. Proc. Natl. Acad. Sci. USA 77: 3504-3508, 1980. PubMed: 6251463 |