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产品简介
购买须知
| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS176889 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | vP-22 |
| Applications： | produces protein thymidine kinase |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | A BglII-BamHI fragment encoding HSV-1 TK (minus the regulatory sequences) was cloned into one of the BamHI sites of an AvaI fragment from the vaccinia L-variant. This construct was then cloned into VTK-79L (ATCC VR-2056) by in vivo recombination. |
| Product Format： | frozen |
| Storage Conditions： | -70°C |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | A recombinant virus containing the HSV-1 Bgl/BamH1 TK-fragment in the vaccinia VTK-79L vector. This clone shows a higher level of TK expression than variants vP-2, vP-4 (ATCC VR-2033), or vP-6 (ATCC VR-2029). A BglII-BamHI fragment encoding HSV-1 TK (minus the regulatory sequences) was cloned into one of the BamHI sites of an AvaI fragment from the vaccinia L-variant. This construct was then cloned into VTK-79L (ATCC VR-2056) by in vivo recombination. |
| Effect on Host： | Cytopathic effects in tissue culture |
| Name of Depositor： | E Paoletti |
| U.S. Patent： | |
| U.S. Patent Number： | |
| References： | Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520 |