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| 产品名称： | pGEM-ratPPT(a) |
|---|---|
| 商品货号： | TS176899 |
| Designations： | pGEM-ratPPT(a) |
| GenBank Number： | L34262 |
| Species： | Rattus norvegicus, rat |
| Depositors： | SL Hofmann |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.5 Tissue: brain Gene product: palmitoyl-protein thioesterase PPT |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.2; EcoRI--3.1, 1.2; PstI--3.4, 0.87; SacI--2.8, 1.5; BglI--2.6, 1.7. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.3, 1.5; BglI--0.5; BglII--1.0, 1.05; EcoRI--1.05; NdeI--1.05; PstI--0.6; PvuII--0.15; SacI--1.45. Constructed from a SstI subfragment of pRatPPT-44. Contains a few nucleotides upstream of the initiation codon, the complete coding sequence, and approximately 500 bp of the 3 untranslated sequence. |
| References： | Camp LA, et al. Molecular cloning and expression of palmitoyl-protein thioesterase. J. Biol. Chem. 269: 23212-23219, 1994. PubMed: 7916016 |