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| 产品名称： | pCu413CUP1 |
|---|---|
| 商品货号： | TS176902 |
| Designations： | pCu413CUP1 |
| Depositors： | S Labbe, DJ Thiele |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4520001411438000 DESCRIPTION OF VECTOR: Intact vector size: 5.452 Type of vector: phagemid Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho Construction: pRS413, CUP1 promoter Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): HIS3, -> replicon: f1, terminator: CYC1, MCS: SpeI...XhoI, -> promoter for expression: CUP1, -> replicon: pMB1 marker(s): ampR, centromere: CEN6 replicon: ARSH4 Vector: pCu413CUP1 (phagemid) Promoters: Promoter for expression CUP1 Construction: pRS413, CUP1 promoter Marker(s):ampR,HIS3 Construct size (kb): 5.4520001411438 Features: marker(s): HIS3 marker(s): ampR promoter for expression: CUP1 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: SpeI...XhoI terminator: CYC1 centromere: CEN6 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI--5.4, SacI/SpeI--5.2, 0.26. Gene CUP1 is the most potent for copper induction. CUP1 promoter can be used to create a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae. |
| References： | Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452 |
| Related Products： | component of:ATCC 87778 |
| Shipping Information： | Distributed: DNA supplied by depositor (frozen) (amount: 100 ng) |