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微信小章| 产品名称： | RH/K34 |
|---|---|
| 商品货号： | TS176935 |
| Organism： | Oryctolagus cuniculus, rabbit |
| Tissue： | peripheral blood |
| Cell Type： | lymphocyte infected with HTLV-I |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates and some adherent cells |
| Biosafety Level： | 2 xa0Cells infected with HTLV-I virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leukemia |
| Gender： | unknown |
| Applications： | The cell line may be used as an infectious human disease model; it can be used to infect rabbits with HTLV-1. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The RH/K34 gamma/delta T cell line was derived from rabbit peripheral blood mononuclear cells (PBMC) by coculture with the rabbit RHT-16 cell line. |
| Clinical Data： | unknown |
| Antigen Expression： | CD5 +/-; CD4 -; CD8 -; MHC class II + Ref   Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938 |
| Receptor Expression： | T cell antigen receptor gamma (TCR), not expressed; interleukin-2 receptor (IL-2R) + Ref Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938 |
| Genes Expressed： | HTLV-I (human T cell leukemia virus),CD5 +/-; CD4 -; CD8 -; MHC class II + |
| Cellular Products： | HTLV-I (human T cell leukemia virus) |
| Comments： | RHT-16 contains integrated HTLV-1 leukemia virus-I (HTLV-1) provirus and sheds intact virus particles.
The RH/K30 (ATCC CRL-2560) and RH/K34 (TS176935) cell lines differ in their potential to cause disease in rabbits.
Injection of the RH/K34 cell line causes human adult T-cell leukemia/lymphoma-like (ATLL) disease that is fatal within 10 days.
However, all rabbits injected with the same or higher doses of RH/K30 survived with a low-grade leukemia that showed evidence of acute rejection.
The cell line may be used as an infectious human disease model; it can be used to infect rabbits with HTLV-1. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. Medium Renewal:xa0Twice per week |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | RM Simpson |
| Deposited As： | rabbit |
| Year of Origin： | 1991 |
| References： | Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938 Zhao TM, et al. Variation in HTLV-I sequences from rabbit cell lines with diverse in vivo effects. Virology 195: 271-274, 1993. PubMed: 8317104 Mahana W, et al. Genes in the pX region of human T cell leukemia virus I influence Vav phosphorylation in T cells. Proc. Natl. Acad. Sci. USA 95: 1782-1787, 1998. PubMed: 9465094 Simpson RM, et al. Experimental acute adult T cell leukemia-lymphoma is associated with thymic atrophy in human T cell leukemia virus type I infection. Lab. Invest. 74: 696-710, 1996. PubMed: 8600320 Chouchane L, et al. Heat-shock proteins expressed on the surface of human T cell leukemia virus type I-infected cell lines induce autoantibodies in rabbits. J. Infect. Dis. 169: 253-259, 1994. PubMed: 7508967 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |