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| 产品名称： | pGCAT-A |
|---|---|
| 商品货号： | TS176957 |
| Designations： | pGCAT-A |
| Depositors： | T Frebourg, O Brison |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5500001907348630 Vector: pGCAT-A (plasmid) Promoters: Promoter T7 Construction: pSB1, pGEM4 Marker(s):ampR Construct size (kb): 4.550000190734863 Features: insert detection: CAT marker(s): ampR promoter: SP6, T7 replicon: pMB1 |
| Applications： | expression vector promoter-cloning vector shuttle vector vector permitting RNA synthesis in vitro |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.3, 0.2; HincII--3.0, 1.5; EcoRI--4.5. A plasmid shuttle vector for investigating promoter and/or enhancer elements. The sequence at the junction of the multiple cloning site and CAT is GAATTAGCTT and at the junction of SV40 and SP6 is GGATCAATTC. The polylinker is in opposite orientation in pGCAT-A (TS176957) and pGCAT-C (ATCC 37641). The BamHI/HindIII fragment of pSB1 containing CAT was cloned into the EcoRI site of pGEM-4 (all ends blunt). Contains the SV40 polyadenylation site and donor and acceptor sites of SV40 small t antigen intron. Has a polylinker upstream of the reporter CAT gene and demonstrates low basal CAT expression in eukaryotic cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Frebourg T, Brison O. Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells. Gene 65: 315-318, 1988. PubMed: 2842234 Thierry Frebourg, personal communication |
| Shipped： | freeze-dried |