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| 产品名称： | pWS8 |
|---|---|
| 商品货号： | TS177010 |
| Designations： | pWS8 |
| GenBank Number： | J04344 |
| Species： | Wound tumor virus |
| Depositors： | DL Nuss |
| Vector： | Construct size (kb): 4.46999979019165 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.47000002861023 Gene product: segment S8, capsomer protein P8 |
| Insert Size (kb)： | 1.470 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal SacI, AccI, HincII and PstI sites. Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--4.65; BamHI--4.65; XbaI--uncut; PstI--4.1, 0.65; EcoRI--4.75; EcoRI/HindIII--3.05, 1.6; BamHI/HindIII--3.05, 1.6. Plasmid must be transformed into a dam- Escherichia coli strain - otherwise XbaI will not cut. Linearize the plasmid with XbaI for in vitro transcription. This is a full-length clone of virus segment S8. This construct gives a transcript with termini identical to the authentic S8 plus-strand sequence (5-GGUAUU---UGAU-3) when linearized with XbaI for transcription and subsequent mung bean nuclease digestion. The cDNA was tailored to a 5-terminal BamHI site followed by the T7 promoter fused to the S8 5-end. The 3-end was tailored to an XbaI site. This construct was cloned into BamHI-XbaI digested pSP64. |
| Classification： | Phytoreovirus |
| References： | Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991 Donald L Nuss, personal communication |