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| 产品名称： | pCry1 |
|---|---|
| 商品货号： | TS177037 |
| Designations： | pCry1 |
| GenBank Number： | M63170 |
| Species： | Mus musculus, mouse |
| Depositors： | R Klemenz |
| Vector： | Construct size (kb): 3.700000047683716 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6940000057220459 Tissue: NIH 3T3 cell line Gene product: crystallin, alpha B Cry1 |
| Insert Size (kb)： | 0.694 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 0.7; BamHI--2.9, 0.35, 0.3; HindIII--2.9, 0.65; PstI--3.7; PvuII--2.4, 0.7, 0.5. The insert includes the following restriction sites (approximate kb from the 5 end): BamHI--0.06, 0.4, 0.41; PvuII--0.2; HindIII--0.63. Includes the complete coding region (nt 27-554), the complete 3 untranslated region, and 27 nt of the 5 untranslated region. The amplification product from the primers AACCGACTCTGCATTCATCTAGCCACAATG and CACTAGTCGACTCGAGT(15) was cloned into the SmaI site. The initiation ATG is at the 3 end of the upstream primer and at the 5 end of the sequence record. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Klemenz R, et al. alphaB crystallin accumulation is a specific response to Ha-ras and v-mos oncogene expression in mouse NIH 3T3 fibroblasts. Mol. Cell. Biol. 11: 803-812, 1991. PubMed: 1846673 |