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| 产品名称： | pTM-N |
|---|---|
| 商品货号： | TS177058 |
| Designations： | pTM-N |
| Depositors： | X Liu |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.8499999046325680 Vector: pTM-N (plasmid) Promoters: Promoter for expression T7 (phi10) Construction: pET-3a,pIN-III-ompA3 Marker(s):ampR Construct size (kb): 2.849999904632568 Features: marker(s): ampR other: sequence for 3 mRNA secondary structure other: sequence for 5 mRNA secondary structure promoter for expression: T7 (phi10) replicon: pMB1 MCS: NcoI...BamHI ribosome-binding site: ompA Shine-Dalgarno signal peptide coding region: ompA |
| Applications： | expression vector vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9; EcoRI--2.9; NcoI--2.9. Expression vector permitting overproduction and secretion of foreign proteins in an unfused state. The ompA signal sequence directs proteins to the periplasmic space. pTM-N differs from pTO-N (GenBank accession M34008) only in the bla (ampR) gene. The PstI site in M34008 has been removed in pTM-N. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Deng TL, et al. A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli: overproduction of bovine pancreatic phospholipase A2. Gene 93: 229-234, 1990. PubMed: 2227436 |
| Shipped： | freeze-dried |