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微信小章| 产品名称： | SU-DHL-1 |
|---|---|
| 商品货号： | TS177091 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node Derived from Metastatic Site: peritoneal effusion |
| Cell Type： | histiocytic |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | large cell lymphoma; diffuse histiocytic lymphoma |
| Age： | 10 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Applications： | The availability of a permanently established human neoplastic cell line as the source of this type C RNA virus should greatly facilitate studies of its biological significance. Evidence has recently been obtained that the virus can induce proliferation of normal human monocytes and histiocytes in vitro. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 88 chromosomes; t(2;5)(p23;q35) translocation |
| Images： |  |
| Clinical Data： | 10 years Caucasian Male |
| Antigen Expression： | Monocyte Marker: CD163+ Lymphoid Marker: CD45- Progenitor Markers: CD10-, CD34- Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45- T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8- B-Cell Markers: CD19-, CD20-, CD21-, CD22- Myelomonocytic Markers: CD11b-, CD11c-, CD13-, CD14-, CD15-, CD33- |
| Receptor Expression： | CFS-1 expressed IL-1-R, IL-2-Rα, IL-6-R, TNFα-R, & c-fms expressed Surface Receptors: Fc, IgMEAC, IgGEA, & E not expressed |
| Oncogene： | c-fms (proto-oncogene), bcl-6+(c-onc) |
| Genes Expressed： | Fusion gene NPM-ALK (p80)+ anaplastic lymphoma kinase (ALK)+ Ig, not expressed |
| Comments： | The cells are surface Ig negative (sIG-). The cells are non-specific esterase, acid phosphatase and oil red O positive. The cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. The cells are reported to be very weakly E - rosette positive. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 1.5 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Interval: As needed. Medium renewal: every 2 to 4 days |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase Freeze medium: Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11, 12 D13S317: 9, 13 D7S820: 10, 13 D16S539: 11, 12 vWA: 15, 17 THO1: 6, 7 Amelogenin: X Y TPOX: 8 CSF1PO: 12 |
| Population Doubling Capacity： | infinite |
| Name of Depositor： | A Epstein |
| References： | Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794 |