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微信小章| 产品名称： | p79-2-23 |
|---|---|
| 商品货号： | TS177094 |
| Designations： | p79-2-23 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Applications： | Lathrop et al (HGM9) have used CEPH families to assign the following linkage group: cen- HP- CTRB- D16S20- pLC9- (APRT, D16S7)- qter. |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.450000047683716 Gene product: DNA Segment, single copy D16S7 |
| Insert Size (kb)： | 1.450 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 1.5; PstI--3.2, 0.86; EcoRI--4.5; HindIII--4.5; SmaI--4.5. Lathrop et al (HGM9) have used CEPH families to assign the following linkage group: cen- HP- CTRB- D16S20- pLC9- (APRT, D16S7)- qter. Derived from cosmid CF33-79. The number of alleles detected is a function of population size and gel resolution; in a study of >1000 unrelated individuals another lab claims to have detected over 100 alleles. Use standard hybridization and washing conditions, with a final wash in 0.1X SSC/0.1% SDS at 65C. |
| References： | Bufton L, et al. A highly polymorphic locus on chromosome 16q revealed by a probe from a chromosome-specific cosmid library. Hum. Genet. 74: 425-431, 1986. PubMed: 2878870 Chimera JA, et al. Population genetics of the highly polymorphic locus D16S7 and its use in paternity evaluation. Am. J. Hum. Genet. 45: 926-931, 1989. PubMed: 2574004 Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986 Michael Litt, personal communication |