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| 产品名称： | Human parainfluenza virus 4a |
|---|---|
| 商品货号： | TS177192 |
| Classification： | Paramyxoviridae, Rubulavirus, Human parainfluenza virus 4 |
| Deposited As： | Parainfluenza 4a |
| Agent： | Human parainfluenza virus 4a |
| Strain： | M-25 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Adapted to NCI-H292 cells from VR-279™. |
| Effect on Host： | CPE, cell degeneration and rounding |
| Recommended Host： | NCI-H292 (ATCC® CRL-1848™) |
| Growth Conditions： | Temperature: 36°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-7 days
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| Name of Depositor： | JC Hierholzer, Centers for Disease Control and Prevention |
| Source： | Throat swab of patient with mild upper respiratory tract illness, 1958 |
| References： | Castells E, et al. NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses. Arch. Virol. 115: 277-288, 1990. PubMed: 2260924 Johnson KM, et al. Studies of a new human hemadsorption virus. I. Isolation, properties and characterization. Am. J. Hyg. 71: 81-92, 1960. PubMed: 14407482 |