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| 产品名称： | YEp351 plasmid in E.coli |
|---|---|
| 商品货号： | TS177283 |
| Designations： | YEp351 plasmid in E.coli |
| Depositors： | A Tzagoloff |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.64 Vector: YEp351 (plasmid) Promoters: Promoter lac Construction: pUC18, pC4B Marker(s):LEU2,ampR Construct size (kb): 5.639999866485596 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: lac replicon: pMB1, 2 micron terminator: none enhancer: none |
| Applications： | YE-type (episomal) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0; XbaI--6.0; PstI--6.0; HindIII--6.0. This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning. pUC18 was digested with NdeI and blunt-ended by filling in. A 2.95 BamHI/XbaI fragment of pC4B was blunt-ended (filled in) and ligated to the pUC18. The 2.95 kb fragment of pC4B includes the LEU2 gene (nt 286-2256 in the final construct, where the A of the ATG of lacZ is number 1) and the 2 micron replication system (nt 2257-3241). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305 Alexander Tzagoloff, personal communication |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Glycerol stock of xa0E. coli containing the plasmid |