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| 产品名称： | pWE2 |
|---|---|
| 商品货号： | TS177334 |
| Designations： | pWE2 |
| Depositors： | M Cockett |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101, ATCC 33694 |
| Vector Information： | Size (kb): 7.014
Vector: pWE2 (phagemid)
Promoters: Promoter for in vitro transcription SP6
Marker(s): G418R,ampR
Construct size (kb): 7.014
Features: marker(s): G418R marker(s): ampR promoter for expression: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: SV40 replicon: f1 replicon: pMB1 MCS: HindIII...ApaI terminator: SV40 polyadenylation enhancer: SV40 |
| Applications： | Expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; SalI--7.0. Having identical cloning sites also facilitates shuttling cDNAs from one vector to another. One of a series of vectors (ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678). Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206 |
| Related Products： | Component of: ATCC 87678 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of xa0E. coli containing the plasmid |