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| 产品名称： | Measles virus |
|---|---|
| 商品货号： | TS177357 |
| Classification： | Paramyxoviridae, Morbillivirus |
| Agent： | Measles virus |
| Strain： | Edmonston |
| Applications： | A CE adapted Edmonston strain has been used for the production of vaccines for human use. Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Measles, Rinderpest and Canine distemper viruses contain a common antigen. |
| Effect on Host： | CPE, syncytial rounding and sloughing |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-14 days |
| Name of Depositor： | JF Enders, Boston Children’s Hospital, Boston, Massachusetts |
| Special Collection： | NCRR Contract |
| Source： | Blood from patient in acute phase of typical measles, Massachusetts, 1954 |
| References： | Enders JF, Peebles TC. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc. Soc. Exp. Biol. Med. 86: 277-286, 1954. PubMed: 13177653 |