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| 产品名称： | pG7/T1 N5-7/U7-T1 |
|---|---|
| 商品货号： | TS177445 |
| Designations： | pG7/T1 N5-7/U7-T1 |
| GenBank Number： | J02636 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 14.39999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 6.699999809265137 Gene product: coenzyme QH2-cytochrome c reductase COR1 |
| Insert Size (kb)： | 6.700 |
| Genotype： | MATalpha ura3-1 cor1-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb) (approximate): EcoRI- 1.0- EcoRI- 0.1- SphI- 0.3- EcoRI- 1.0- XhoII- 1.6- EcoRI- 0.3- XbaI- 1.1- XhoII. Restriction digests of the clone give the following sizes (kb): SmaI/SalI--6.2, 4.2, 4.1; SalI--10.6, 4.1; EcoRV--14.4; XbaI--9.3, 5.2. The smallest subclone capable of complementing a cor1-1 mutation was a 3.0 kb XhoII fragment. This region contains a continuous 1.368 kb open reading frame encoding a protein of 456 amino acids with a molecular weight of 50 kDa. The probe, pG7/T1, has been deposited in Escherichia coli RR1 as TS177445 and in Saccharomyces cerevisiae N5-7/U7 as ATCC 66316. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Tzagoloff A, et al. Assembly of the mitochondrial membrane system. Characterization of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase. J. Biol. Chem. 261: 17163-17169, 1986. PubMed: 3023384 |