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| 产品名称： | HPalpha2GEN 5.5 BamHI fragment |
|---|---|
| 商品货号： | TS177459 |
| Designations： | HPalpha2GEN 5.5 BamHI fragment |
| Species： | Homo sapiens, human |
| Depositors： | BK Kobilka |
| Vector： | Construct size (kb): 8.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 5.5 Tissue: platelet Gene product: adrenergic, alpha-2A-, receptor( Adrenergic, alpha 2A, receptor, human adrenergic alpha2A receptor, alpha2A-adrenergic receptor) ADRA2 |
| Insert Size (kb)： | 5.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains SmaI (5), KpnI (1), SacI (4), NcoI (1), PstI (4), HindIII (2), and SphI (1) sites. Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 2.7; HindIII--3.6, 3.5, 1.3; PstI--4.9, 2.2, 0.9; SalI--8.2; EcoRI--8.2. The 2.0 kb BamHI/PstI fragment detects the following human genomic restriction fragments (kb): SfiI--225; SalI--380, 510; SacII, PvuI, MluI--greater than 2000; SfiI/SacII--225; SfiI/MluI--225; SfiI/PvuI--225. The 2.0 kb BamHI/PstI fragment comes from the 5 untranslated region. The original published sequence in J. Biol. Chem. 265: 17307-17317, 1990 is incorrect. The GenBank record is correct. The 2 kb BamHI/PstI fragment is a unique sequence in the genome. The 0.95 kb fragment hybridizes to a 1.8 kb PstI fragment apparently mapping to chromosome 2 and a 5.9 kb PstI fragment apparently mapping to chromosome 4. The 2.0 kb BamHI/PstI fragment detects a 3.0 kb PstI fragment specific to mouse chromosome 19. |
| References： | Yang-Feng TL, et al. Chromosomal organization of adrenergic receptor genes. Proc. Natl. Acad. Sci. USA 87: 1516-1520, 1990. PubMed: 2154750 Kobilka BK, et al. Cloning, sequencing, and expression of the gene coding for the human platelet alpha2-adrenergic receptor. Science 238: 650-656, 1987. PubMed: 2823383 Brian K Kobilka, personal communication |