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| 产品名称： | pZB4 plasmid in Zymomonas mobilis, CP4 |
|---|---|
| 商品货号： | TS177469 |
| Designations： | pZB4 plasmid in Zymomonas mobilis, CP4 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | Midwest Research Institute, M Zhang, Midwest Research Institute |
| Applications： | produces protein transaldolase B produces protein transketolase 1 (TK 1) transketolase 1 produces protein xylose isomerase glucose isomerase produces protein xylulokinase |
| Vector： | Vector: pACYC184 Vector type: plasmid Vector size (kb): 4.2 Markers: tetR Construction: p15A, pSC101, Tn9 Replicon: p15A |
| Insert： | Insert size (kb): 2.8 Gene: transketolase 1 (talB) DNA: genomic Source: Escherichia coli |
| Insert Size (kb)： | 2.800 |
| Media： | Zymomonas medium w/
glucose (20 g/L)
yeast extract (10 g/L)
KH2PO4 (2 g/L)
tetracycline (10 mcg/ml)
pH6.0
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried Z. mobilis containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--4.2, 2.8. The GAP/xylA/xylB operon may be recovered as a 4.1 kb NotI fragment. This strain containing pZB4 is useful for fermenting glucose and pentoses (xylose), produced by hydrolysis of cellulose and hemicellulose, to produce ethanol. The xylA/xylB operon is controlled by the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The talB/tktA operon is controlled by the Z. mobilis enolase (ENO) promoter. The GAP and ENO promoters are in a head-to-head orientation. Plasmid contains the GAP-xylA/xylB operon from pGapXKH in a clockwise orientation and ENO-tal/tkt operon from EnoTAL/TKT in a counterclockwise orientation. This plasmid is shipped in Zymomonas mobilis CP4. It should be grown in Zymomonas media (20 g glucose, 10 g Yeast extract, 2 g KH2PO4 in one liter of distilled water) with 10 ug/ml tetracycline at 30C in an anaerobic gas mixture of 80%N2-10%CO2 - 10% H2. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Picataggio SK, et al. Recombinant Zymomonas for pentose fermentation. US Patent 5,514,583 dated May 7 1996 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |