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微信小章| 产品名称： | Murine leukemia virus |
|---|---|
| 商品货号： | TS177470 |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| Deposited As： | Murine erythroleukemia virus |
| Agent： | Murine leukemia virus |
| Strain： | Rauscher |
| Applications： | The R-SFFV clone can be rescued by superinfecting the host cells with Rauscher MuLV. Several weeks PI the cells will express R-SFFV and R-MuLV which can be injected into susceptible mice pretreated with phenyl hydrazin. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Biologically cloned NIH/3T3s infected with biologically cloned R-SFFV from Rauscher erythroleukemia cell line |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | The R-SFFV clone can be rescued by superinfecting the host cells with Rauscher MuLV. Several weeks PI the cells will express R-SFFV and R-MuLV which can be injected into susceptible mice pretreated with phenyl hydrazin. |
| Effect on Host： | No, in vitro effects: cytopathic effects in NIH/3T3 cells Yes, in vivo effects: rescued R-SFFV causes proliferation of hematopoietic stem cells of mice in vivo or in vitro |
| Recommended Host： |
Mus musculus mouse |
| Growth Conditions： | Temperature: 37°C Incubation: 3 days |
| Name of Depositor： | D Kabat |
| Special Collection： | ATCC |
| Source： | Biologically cloned NIH/3T3s infected with biologically cloned R-SFFV from Rauscher erythroleukemia cell line |
| References： | Machida CA, et al. Reduced leukemogenicity caused by mutations in the membrane glycoprotein gene of Rauscher spleen focus-forming virus. J. Virol. 49: 394-402, 1984. PubMed: 6319740 Ruta M, Kabat D. Plasma membrane glycoproteins encoded by cloned Rauscher and Friend spleen focus-forming viruses. J. Virol. 35: 844-853, 1980. PubMed: 7420541 Ruta M, et al. A murine leukemia virus mutant with a temperature-sensitive defect in membrane glycoprotein synthesis. Cell 16: 77-88, 1979. PubMed: 421271 Stephenson JR, Aaronson SA. Characterization of temperature-sensitive mutants of murine leukemia virus. Virology 54: 53-59, 1973. PubMed: 4123462 |