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| 产品名称： | YEp6 pRB15 |
|---|---|
| 商品货号： | TS177484 |
| Designations： | YEp6 pRB15 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.9000000953674320 Vector: YEp6 (plasmid) Construction: pBR322, Saccharomyces cerevisiae, 2 micron ori Marker(s):ampR,HIS3 Construct size (kb): 7.900000095367432 Features: marker(s): ampR, HIS3 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector |
| Comments： | A general purpose episomal Saccharomyces cerevisiae/Escherichia coli shuttle vector. YEp6 is deleted of approximately 4 kb of 2 micron sequences and 200 bp of pBR322. It is stably maintained at high copy number in cir+ strains. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |