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| 产品名称： | pAVE2 pAVE-2 |
|---|---|
| 商品货号： | TS177523 |
| Designations： | pAVE2 pAVE-2 |
| Depositors： | HA Barrera Saldana |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.395 Vector: pAVE2 (plasmid) Promoters: Promoter hCMV Construction: pAVE1 (ATCC 37838), pMThGH Marker(s):ampR Construct size (kb): 3.395 Features: marker(s): ampR promoter: hCMV replicon: pMB1 terminator: hGH enhancer: hCMV |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII-3.5; BamHI--3.5; EcoRI--3.5; SmaI--3.5. Commercial use of the human cytomegalovirus promoter/enhancer has been patented on behalf of B. Fleckenstein and W. Schaffner by the Beringwerke. Interested parties should contact the patent holders before planning a commercial application. A shuttle vector for transient expression of cDNA inserts in mammalian cells. Constructed by inserting a 0.62 kb SmaI/EcoRI fragment from pMThGH containing part of exon 5 and 3 untranslated regions of the human growth hormone gene into the SmaI/EcoRI sites of pAVE1 (ATCC 37838). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ramirez-Solis R, et al. New vectors for the efficient expression of mammalian genes in cultured cells. Gene 87: 291-294, 1990. PubMed: 2158929 |
| Shipped： | freeze-dried |