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| 产品名称： | Angio-Ready™ Angiogenesis Assay System |
|---|---|
| 商品货号： | TS177561 |
| Organism： | Homo sapiens, human |
| Tissue： | aortic and adipose |
| Cell Type： | aortic endothelial and adipose mesenchymal stem cell |
| Product Format： | frozen Ten 1 mL vials of cells Five 200 mL liquid medium kit |
| Morphology： | endothelial- and mesenchymal-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells containing SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 23 (TeloHAEC-GFP); N/A for hTERT-MSC |
| Gender： | both female |
| Ethnicity： | both Caucasian |
| Applications： | In vitro angiogenesis assay for drug screening, toxicity, pathway research, and gene function study |
| Shipping Information： | 10 frozen 1 mL vials of cells (Angio-Ready™ Cells ACS-2007™) and five 200 mL liquid medium kit, Angio-Ready™ Angiogenesis Medium with VEGF Supplement (ACS-2008), shipped on dry ice |
| Storage Conditions： | cells at -130ºC; medium at -20ºC |
| Karyotype： | normal |
| Derivation： | Derived from aortic and adipose tissue; a proprietary mix of TeloHAEC-GFP (ATCC CRL-4054) and ASC52telo (ATCC SCRC-4000) hTERT-immortalized cells |
| Antigen Expression： | CD31 and CD90 |
| Oncogene： | may contain viral sequences |
| Comments： | For details regarding Angio-Ready Angiogenesis Medium with VEGF Supplement see ATCC® ACS-2008 |
| Complete Growth Medium： | Angio-Ready™ Angiogenesis Medium with VEGF Supplement (ATCC® ACS-2008) |
| Seeding Density： | 7.5 x 104 per well of a 96-well plate (for other size plates refer to protocol) |
| Cryopreservation： | 90% FBS + 10% DMSO |
| Cells per Vial： | 7.5X106 |
| Volume： | Ten 1 mL vials of cells Five 200 mL liquid medium kit |
| COI： | Human |
| Sterility Tests： | none detected |
| Viral Testing： | none detected |
| Functional Tests： | Pass – tubular formation assay |
| Population Doubling Time： | N/A |
| Name of Depositor： | ACS |
| Year of Origin： | N/A |
| Year of Deposit： | N/A |
| References： | Hsu CW, et al. Identification of approved and investigational drugs that inhibit hypoxia-inducible factor-1 signaling. Oncotarget 7(7): 8172-8183, 2016. PubMed: 26882567 |