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| 产品名称： | BNsp-Neo-T |
|---|---|
| 商品货号： | TS177608 |
| Designations： | BNsp-Neo-T |
| Depositors： | LH Van Der Ploeg, M Lee |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.1999998092651370 Vector: BNsp-Neo-T (plasmid) Promoters: Promoter PARP Construction: pUC18, pSV2neo, BNsp-CAT Marker(s):G418R,ampR Construct size (kb): 6.199999809265137 Features: marker(s): ampR, G418R promoter: PARP replicon: pMB1 |
| Applications： | integrating vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII, BamHI--3.5, 2.7; EcoRI, BglII--5.6, 0.6; BamHI--6.2; MluI--6.2. Linearizing with MluI before transfection is essential for efficient integration. The MluI site is in the center of the tubulin intergenic sequence. Shuttle vector for integration by homologous recombination targeted to the beta- alpha-tubulin intergenic region of Trypanosoma brucei. Constructed from the PARP promoter and 3 splice site from BNsp-CAT, neo (BglII/BamHI fragment) from pSV2neo, and a tubulin intergenic region (639 bp, blunted EcoRI/BglII) inserted into the SmaI site 3 to the termination codon of neo. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lee MG, Van der Ploeg LH. Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei. Science 250: 1583-1587, 1990. PubMed: 2177225 |
| Shipped： | freeze-dried |