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| 产品名称： | p426 TEF |
|---|---|
| 商品货号： | TS177687 |
| Designations： | p426 TEF |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.3590002059936520 DESCRIPTION OF VECTOR: Intact vector size: 6.359 Type of vector: plasmid Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI Other unique sites: SacI Construction: pRS426 (ATCC 77107) Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): URA3, ->, 200-1300 terminator: CYC1, ->, 2004-2264 MCS: XhoI...XbaI, ->, 2265-2339 promoter for expression: TEF, marker(s): ampR, replicon: 2 micron, ->, 4889-6229 Vector: p426 TEF (plasmid) Promoters: Promoter for expression TEF Construction: pRS426 (ATCC 77107) Marker(s):URA3,ampR Construct size (kb): 6.359000205993652 Features: marker(s): URA3 marker(s): ampR promoter for expression: TEF replicon: 2 micron MCS: XhoI...XbaI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.5; SacI/XBaI--3.2, 2.7, 0.44; XhoI--6.5. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |